ATP LLD (pM) ATP LLD (moles) SpectraMax i3x, luminescence . Not compatible with Home Edition. ai; ImageXpress Pico; IN Carta Image Analysis Software; CLONE SCREENING. 302 low ref light. SpectraMax i3x; SpectraMax iD3 / iD5; SpectraMax M Series; SoftMax Pro Software; CELLULAR IMAGING. EUROPE. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. Results were plotted as ratio of green/red vs. to View Figures. Figure 4. AlphaScreen assays are compatible with our SpectraMax i3x, SpectraMax i3, and SpectraMax Paradigm Multi-Mode Microplate readers with the Alphascreen Detection Cartridge options. Detection cartridge modularity allows for easy addition of new read modes within minutes without. Thetypeof luciferase(Renilla. 12911151) 0. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast. I am a person who can handle a range of laboratory and data management tasks with precision and accuracy. The SpectraMax i3x reader allows you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one system. Explore more about the SpectraMax i3x Multi-Mode Plate Reader, a user-upgradeable detection modules allow you to expand the capabilities of your microplate reader when your research requires it. com Check our website for a current listing of worldwide distributors. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. D 1 1. The SoftMax Pro Software provides integrated instrument control. Additionally, user-installable options allow the SpectraMax i3 System to grow with your changing application needs to fulfill and go beyond the standard reader applications, protecting your initial investment. Supported Windows Operating System: See release notes. Microplate Reader Shelf. Molecular Devices SpectraMax i3x Multi-Mode Microplate Platform. Request a Quote for This Setup. . HTRF-compatible SpectraMax Multi-Mode Readers. Register to download your free eBook today. Read modes. SpectraMaxMiniMaxImagingCytometerInstallationGuide 10 5024111D AttachingtheCytometertotheMicroplateReader Prepareaworkareathatisapproximately130 cm(51. Get more information about Fluorescence Polarization SpectraMax Detection Cartridges, which offer great sensitivity for SpectraMax i3x and Paradigm Systems. This update is applicable for users of the SpectraMax® i3x Multi-Mode Detection Platform and SpectraMax® Paradigm® Multi-Mode Microplate Reader with the ScanLater Western Blot (WB) Detection Cartridge and the SpectraMax® iD5 Multi-Mode Microplate Reader: SMP 7. 과 같은 Parameter를 검사하여 평가할 수 있습니다. KEY FEATURES • Combine cellular imaging and microplate assays into a workflow • Eliminate cell staining for cell counting and confluency with StainFree TechnologyThe SpectraMax® M5 utilizes a single monochromator design for UV/VIS Absorbance, allowing the user to maximize sensitivity and tunability, and also uses patented PathCheck® Pathlength Measurement Technology: the only temperature independent pathlength correction for microplates. Also review the Maintenance and Troubleshooting chapter of the user guide for your model plate reader. No accessories are needed and SoftMax® Pro. . Page 38 SpectraMax iD3 Multi-Mode Microplate Reader User Guide Bottle Holder The bottle holder is mounted on two knobs for ease of installation, cleaning, and replacement. Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. Videos & Webinars. Features technology from the SpectraMax i3x and MiniMax 300 Imaging Cytometer. The heating element design of the category 1 microplate readers, such as the SpectraMax i3x Multi-Mode Microplate Reader, applies a dual area sensor for temperature control. . Molecular Interactions. Find regional support. HTRF IP1 standard curve measured on the SpectraMax i3x (green circles) and SpectraMax iD5 (red circles) readers. SpectraMax Paradigm Microplate Reader General specifications User-upgradeable Multi-Mode Detection Platform Technical specifications Optimized Guaranteed* Fluorescence Intensity (FI) (Fluorescein) 96-well (200 µL) 0. In order to calculate a humification index (HIX), spectra were obtained with an excitation wavelength of 254 nm over the emissions waveband of 300–480 nm at increments of 5 nm (Ohno 2002. com The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. 4. Molecular Devices Spectramax 340 Absorbance Plate Reader (1997) Molecular Devices SpectraMax i3x Multi-Mode Microplate Reader (2016) Vernier UV-VIS Spectrophotometer (5) Vernier VIS Spectrophotometer (30) Infrared Spectroscopy. Antigen Quantitation Protocol. SpectraMax iD5:SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. compound concentration, and an IC 50 value of 21. Adding time resolved fluorescence, HTRF® or western blot detection simply requires the user to exchange a detection module. The SpectraMax® i3 Platform from Molecular Devices® is a multi-mode detection system that evolves with your future needs and offers an unlimited breadth of application possibilities. Data were analyzed with SoftMax ® Pro Software and graphed using a 4-parameter curve fit. We would like to show you a description here but the site won’t allow us. Sensitive TRF detection of two simple, mix-and-measure assay workflows. The SpectraMax i3x is a multi-mode microplate reader that measures absorbance, fluorescence, and luminescence with user-configurable detection modules for western. Concentrating the excitation light on the sample through label specific optics, high levels of sensitivity and performance are reached. 2. A: percent GFP-positive cells calculated using the classification feature in the software. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. The solutions were thoroughly mixed and then incubated in the dark at 20°C for 2 h. . 2 GxP Software is the latest, most secure software to achieve full FDA 21 CFR Part 11 and EudraLex Annex 11 compliance with. This eBook shows how to see more and do more with an imaging cytometer that enables rapid imaging and cell analysis. HeLa cells were plated at 15,000 cells per well in 100 µL. Contact Us Phone: +1. 99. SpectraMax i3/i3x Multi-Mode Microplate Reader SoftMax Pro Software 0 500 1000 1500 2000 2500 3000 0 1´ 106 2´ 106 3´ 106 dsDNA (pg/we l) R F U 02 4 6 8 1 0 2. ; India imports most of its Spectramax from United States, China and Austria and is the largest importer of Spectramax in the World. . Catalog excerpts. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. 5mM EDTA, 1% NP40,Explore a wealth of applications with the future-ready SpectraMax i3x. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. i3x single use: $35: $52. The SpectraMax® i3x reader allows you to. . Spectramax I3x, supplied by Danaher Inc, used in various techniques. 99 to $1999. Specifically, we present neurite. CHO M1WT3 cells are Chinese hamster ovary cells stably transfected with the M1 muscarinic G q -coupled receptor. The microplate was then placed in a SpectraMax i3x reader and incubated at 37°C for the duration of the kinetic measurement. Overview of Western Blot Cartridge. Pick the best speed, sensitivity, and price for your needs. SpectraMax® i3x. The plate was then read on the SpectraMax iD5, SpectraMax i3x, and SpectraMax M5e readers using the settings in Table 1. 9000-0756. 1. Dual auto injector cartridges for the SpectraMax® i3x Multi-Mode Microplate Detection Platform allows you to expand your research capabilities to include flash based applications, including dual luciferase and ATP assays. 7 mm for all shake intensities. Use the 3. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast. 313 reference gain check fail. AlphaScreen. Controls containing all reaction components with either DI water or a fluorescent standard in place of the fluorescent substrate were run alongside the experimental samples. The SpectraMax i3x reader provides theThe SpectraMax® i3x multi-mode microplate reader is a configurable, versatile microplate reader that evolves with your future needs and allows you to investigate every aspect of a cellular pathway. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. The SpectraMax® DuoLuc™ Reporter Assay Kit enables highly sensitive quantitation of both firefly and Renilla luciferases in mammalian cells at a great price. The SpectraMax i3 Multi-Mode Detection Platform is a versatile instrument that can perform various assays and measurements on microplates and cuvettes. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. A SpectraMax® i3x was used to determine fluorescence with an excitation wavelength of λ = 360 nm and emission at λ = 460 nm. The SpectraMax® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. The corrected absorbance is within 5% of the. SpectraMax i3 Install Guide; SpectraMax i3; SpectraMax i3x; SpectraMax iD3; SpectraMax iD5; SpectraMax Mini; SpectraMax Mini Install Guide; SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. The SpectraMax® Quant™ dsDNA Assay Kits are designed for fluorescence-based dsDNA quantitation across a broad range of concentrations. ZERO BIAS - scores, article reviews, protocol conditions and moreStructured Review. 58% for the detection module and 2. Danaher Inc spectramax i3x Spectramax I3x, supplied by Danaher Inc, used in various techniques. This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. 0112-0115J 9 Chapter1:Introduction TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-ModeSpectraMax i3x and iD5 readers can be equipped with an HTRF detection cartridge or Enhanced TRF module and filters, respectively. Nuclear Receptor. Expanded read mode capabilities Equipped for high performance, the SpectraMax iD5 is HTRF®-certified, DLReady, and SpectraMax® DuoLuc™certified. Description 1. Cet appareil ISM est confomre à la norme NMB-001 du Canada. Get more information about Fluorescence Polarization SpectraMax Detection Cartridges, which offer great sensitivity for SpectraMax i3x and Paradigm Systems. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. ZERO BIAS - scores, article reviews, protocol conditions and moreThe SpectraMax MiniMax 300 Imaging Cytometer option can be added at any time to the SpectraMax i3x System, providing an expandable platform that evolves with your changing needs. The. It can be upgraded with modules such as imaging cytometer, western blot imager, and injectors to expand its capabilities. Quick imaging and analysis of cells gives you a front-row view of the phenotypic changes that accompany. Additionally, user-installable options allow the SpectraMax i3 System to grow with your changing application needs to fulfill and go beyond the standard reader applications, protecting your initial investment. After 2 days of incubation, LUC and REN activities were measured using a SpectraMax ® i3x Multi-Mode detection platform (Molecular Devices, USA) with a Dual-Luciferase Reporter Assay Kit (Promega. . GM3000 Register or Sign In. A SpectraMax ® i3x was used to determine uo-rescence with an excitation wavelength of λ = 360 nm and emission at λ = 460 nm. 13 There are a fairly limited number ofSARS-CoV-2 variants of concern (VOCs) that increase transmission or disease severity or reduce diagnostic or vaccine efficacy continue to emerge across the world. Contact Local Team. The experiments were performed in triplicates and the spectral reading was recorded using Spectramax i3x microplate reader. 9 x 10-15. Figure 1. SpectraMax iD3 and iD5 Register or Sign In. Molecular Devices SpectraMax ® i3x MultiMode Microplate Reader, in combination with luminescent assays for cell viability and cytotoxicity, provides a sensitive and rapid way to measure the number of viable cells in culture and quantify the cytotoxic effects of experimental treatment. Ribonuclease (RNase) R digestion assay For identifying the stabilization of circVRK1, 2 μg isolated RNA from HBMECs was digested by RNase R (3 U/μg; RNR07250, Geneseed, Guangzhou,. The SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. These instruments often feature additional detection modes in addition to fluorescence intensity, such as UV-vis absorbance, fluorescence polarization, and time-resolved. Expanded dynamic range Engineered for performance with spectral fusion illumination for increased sensitivity across the entire excitation range. . For SpectraMax iD5, i3x, and Mini readers, the read height setting should be optimized for the microplate used. The SpectraMax® i3x Multi-Mode Microplate Reader evolves with your future needs and offers an unlimited breadth of application possibilities. 660-665 Eskdale Road. Ease of use Because microplate readers are meant to shoulder routine and time-consuming tasks for researchers, ease of use is an important feature. 3. +44-118-944-8000. The CellTiter-Glo assay from Promega uses luciferase. . NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. 0. . With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. The possibility of user upgrading the system with an application cartridge and an imaging cytometer makes this device the most flexible microplate. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. the SpectraMax iD3 is a fantastic plate reader for a wide range of assays. A 1:2 dilution series of BSA was prepared in PBS, starting at 100 mg/mL, in LoBind microcentrifuge tubes. These robust readers have been placed in labs from Antarctica to the. Molecular Devices社の製品: 吸収度マルチモードマイクロプレートリーダー SpectraMax® i3x に関するすべての情報をご覧ください。. Description 1. 세포신호전달은 세포가 환경에 반응하고 다른 세포와 소통할 수 있게 합니다…. Unlimited breadth of. spectramax i3x - by Bioz Stars , 2023-11. The SpectraMax i3x reader grows with you as you continue to advance your research, enabling you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one system. Table 3 Optimal instrument settings for Valita Titer Plus assay Fluorescence Polarization measurement on Molecular Devices microplate readers. Insert the snorkel into the bottle and then insert the snorkel into the snorkel clamp. QuickSync. For more information on the SpectraMax i3x Multi-Mode Microplate Reader System, please click here. In this product video, see how the SpectraMax M5 quickly reads samples. Background subtraction was not necessary. Spectramax I3x, supplied by Danaher Inc, used in various techniques. The SpectraMax ® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. Replicate values were averaged and imported into Design-Expert 8. Find regional support. . ValitaTiter Plus settingsContact our support team with your questions regarding Molecular Devices products and applications. Binding and Inhibition. Explore more about Optimized settings for Transcreener TR-FRET assay on SpectraMax iD5 and SpectraMax i3x readers. The SpectraMax® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. . SpectraMax i3x and Paradigm readers require the FP-FLUO detection cartridge. Spectranet customer portalSpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112-0115 Rev. . . 012 fmol 0. SpectraMax i3xは、吸光・蛍光・発光スペクトル測定のためのマルチモードマイクロプレートリーダーです。. 在这里，我们展示了如何使用SpectraMax®i3x和 SpectraMax®iD5多功能微孔板读板机应用HTRF技术进行 Gq-偶联受体的分析。G蛋白偶联受体的分析主要通过检测 cAMP和IP3这两条信号转导通路调控介导的细胞内钙离子水 平的升高。Cisbio公司的IP-One Gq试剂盒通过检. Data from Analyst®, and FLIPR® systems can also be easily imported and analyzed. +44 118 944 8000. Page 1 SpectraMax M2/M2 user guide ® A MULTI-DETECTION MICROPLATE READER WITH TWO-MODE CUVETTE PORT. SpectraMax i3x Multi-Mode Detection Platform. Shaking and incubations were performed using an on-deck orbital shaker. This technique allows for quality control and assurance when performing surface functionalization of nanoparticles. Finally, their fluorescence was measured with 365-nm excitation and 450-nm emission filters using a microplate fluorometer (SpectraMax i3x, Molecular Devices, San Jose, CA, USA) (Saiya-Cork et al. STAKMAX. Bioz Stars score: 98/100, based on 1 PubMed citations. detection cartridge LUM96: 59. Our microplate readers and software are the industry’s most cited and have empowered life science researchers to advance protein and cell biology. Fluorescence measurements of the soil solutions and enzyme activities were made using a microplate reader (SpectraMax i3x, Molecular Devices, USA). Bioz Stars score: 98/100, based on 1 PubMed citations. Qualify the absorbance performance of SpectraMax® iD3, iD5, i3, i3x, M2, M2e, M3, M4, M5, M5e, Plus 384, ABS, ABS Plus, and FlexStation 3 readers. The SpectraMax MiniMax 300 Imaging Cytometer option is a field-upgradeable option for the SpectraMax i3/i3x Multi-Mode Detection Platform that offers cellular imaging capability. Research Article AntioxidantActivity,PolyphenolicContent,andFT-NIR AnalysisofDifferentAspiliaafricanaMedicinalPlantTissues DenisOkello ,1,2,3YuseongChung,1HyoseonKim. Register to download your free eBook today. Cell viability는 세포막 Integrity 또는. ai; ImageXpress Pico; IN Carta Image Analysis Software; CLONE SCREENING. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. FTIR Spectrometer: Shimadzu IRSpirit (2018)The plate was read on the SpectraMax i3x Multi-Mode Microplate Reader. 2 or higher,. to View Figures. DNA/RNA concentration is commonly estimated by dividing the A260 value by the 1-cm absorptivity value (or multiplying by its reciprocal). Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. This document is copyright protected and any reproduction of this document, in whole or any part, is strictly prohibited, except as Molecular Devices may authorize in. Cell counters for accurate and. . Prime function 20 amol. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Both Microplate Optimization and Read Height Adjustment were. 99 to $1999. Expand your lab’s capabilities to include flash applications such as dual luciferase and ATP assays with the SpectraMax® i3x injector module with SmartInject® Technology ensuring equal mixing across the plate for high-precision experiments. Find our technical support information along with contact details. The information is below the horizontal line. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Danaher Inc spectramax i3x. Figure 2-23: Bottle holder To install the bottle holder: 1. 0001 0. SpectraMax M3 Plate Read, Plate Map, Data Analysis and Export Setup pg. The SpectraMax ® i3x Platform from Molecular Devices is a multi-mode microplate reader that evolves with your future needs and offers an unlimited breadth of application possibilities. One sensor measures the temperature of the top chamber heating foil, and another is used to measure the bottom chamber heating foil. Viewing this answer requires an authorized account. Microplate Readers SpectraMax. The SpectraMax® i3 Platform from Molecular Devices® is a multi-mode detection system that evolves with your future needs and offers an unlimited breadth of application. Molecular Devices SpectraMax i3x Multi-Mode Detection Platform. Figure 1. Avantor®, a Fortune 500 company, is a leading global provider of mission-critical products and services to customers in the biopharma, healthcare, education & government, and. Microplate and read height should be optimized for each new lot of microplates or assay volume used (use the Show Pre-Read Optimization options in the instrument settings in SoftMax Pro Software). Failed to load PDF file. 98 / 100 stars. Italic and boldface type are used for emphasis. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. The plate was shaken and incubated in the dark for 10 minutes. Restart the system. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. A SpectraMax® i3x was used to determine fluorescence with an excitation wavelength of λ = 360 nm and emission at λ = 460 nm. ，高内涵成像分析技巧——3D功能展示1-3D预览、共定位、测距，SpectraMax Mini 多功能微孔板读板机带你自信地迈出第一步！ ，多功能酶标仪 SpectraMax i3x 之动力学检测法的设置，iD5注射器卡盒操作视频 - 20201228_8，SpectraMax iD 系列触控屏 overview，【网络讲堂】高内涵. SpectraMax® Microplate Readers and SoftMax® Pro Software. Annual recertification of your validation plates ensure that they meet specifications and give you confidence in the performance of your instrument. Modularity does provide some measure of forward vision into upcoming or future experimental needs. They include the SpectraMax® iD3 Multi-Mode Microplate Reader, SpectraMax® L Microplate Reader, SpectraMax® i3x Multi-Mode Microplate Reader, and SpectraMax® M5 Multi-Mode Microplate Reader. Time-. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. Spectranet customer portalThe SpectraMax i3x measures monochromator-based absorbance, fluorescence and luminescence with a future-ready platform that can evolve with the researcher’s needs. . MC38 in vivo tumor model in hPD-1. **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. The SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. 1 software Download page. 1. Promega. Other technologies such as imaging. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,MDC instruments including: SpectraMax®, VersaMax®, VMax®, EMax®, Gemini, ThermoMax®, and FlexStation®. GM2000 Register or Sign In. SpectraMaxi3Multi. It can be upgraded with. SpectraMax i3x 和 SpectraMax iD5 微孔板读板机上使用 SoftMax Pro 软件中的预设方法进行测量(有关仪器特定设 置，请参见表 2 和表 3 )。在 SpectraMax i3x 和 SpectraMax iD5 读板机上进行了微孔板优化和读取高度调整，以确保最佳 的检测灵敏度和动态范围。 数据分析Spectramax i3x fluorescence plate reader to measure fluo-rescence resonance energy transfer (FRET) signals from cell lines expressing CFP-YFP FRET sensors for glucose and ATP, as these sensors give large changes in FRET ratio in response to appropriate conditions, and are expressed in cells at high levels. The intensity of the emitted light can be measured easily with the SpectraMax i3x reader. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax ® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. The soil suspension (600 µL) and the substrate (L-3,4-dihydroxyphenylalanine, DOPA, 150 uL, 25 mmol L -1 ), were mixed and then added to. Itemploysa luciferaselabel(where“label”generallymeansfusedbytheuseofexpressionvectors)forthe oneproteinandaGreenFluorescentProtein(GFP)variantlabelfortheother. 305 reference level saturation. The plate was shaken and incubated in the dark for 10 minutes. Get a front row view of your cells. The SpectraMax i3x microplate reader measures absorbance, luminescence and fluorescence as standard, and provides the flexibility to add novel. GFP expression imaged on the cytometer. 2 software (Stat-Ease) to identify factors that have significant effects on cell growth or IgG production, either alone or in combination with. . 100 fmol 384-well (75 µL) 0. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Published Dec 16, 2020 | Updated Oct 10, 2023. SpectraMax i3x readers. At each time point, the samples were shaken and fluorescence was measured for SEP (488-nm excitation and 530-nm emission) and. By measuring optical pathlength, it eliminates the last major distinction between microplate readers and spectrophotometers. 315 can't find zero order. SpectraMax® i3x Multi-Mode Detection Platform; SpectraMax® i3 Multi-Mode Detection Platform; SpectraMax® ID3 Multimode Microplate reader; SpectraMax® ID5 Multimode Microplate reader; SpectraMax® Paradigm® Multi-Mode Microplate Reader; SpectraMax® Mini Multi-Mode Microplate Reader (software version 7. ImageXpress Confocal HT. 312 gain calibration failed. For each assay kit, a preconfigured protocol is included in SoftMax Pro Software to facilitate the acquisition and analysis of data for a simplified workflow. The SpectraMax i3x reader allows you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one system. user upgradeable. Actual shake speed is based on the microplate format. SpectraMax Paradigm Cisbio HTRF Detection Cartridge User Guide 5008539 A 5 Laser Light Laser Specifications The SpectraMax® Paradigm® Multi-Mode Detection Platform is rated a Class 1 Laser Product because it houses one or more Laser Modules whereas the laser light is not accessible. SpectraMax i3, i3x, and iD5 readers. . StakMax Handling Stacker Base Unit. Both readers demonstrated their ability to detect the HTRF signal from the terbium donor and red acceptor combination, and they could be used for saturation studies using the Tag-lite technology. . It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. 7. The SpectraMax i3x reader allows you to unravel the mysteries of science by exploring Download SoftMax® Pro 7. user upgradeable. • SpectraMax i3x Injector Cartridge with SmartInject™ Technology • Expand your research capabilities to include flash-based . The SpectraMax M2 and M2e systems provide the multi detection microplate readers with dualmonochromators, dual-mode cuvette ports, with top & bottom reading. Description 1. SpectraMax i3x Multi-Mode Microplate Reader; SpectraMax MiniMax 300 Imaging Cytometer *Here, the NucView 488 with DMSO formulation was used. 0. . See Unpacking the SpectraMax Paradigm Instrument on page 1. Sensitive TRF detection of two simple, mix-and-measure assay workflows. We would like to show you a description here but the site won’t allow us. 2-µL or 4-µL samples were pipetted onto a 64-well SpectraDrop slide in quadruplicate, following the plate map in Figure 2. Version 7. Price from $9. The SpectraMax® i3x offers a wealth of applications; it can measure fluorescence, absorbance and luminescence, and its modular upgrades enable western blotting, imaging cytometry and injectors. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Spectramax i3 manualSpectramax i3 manual Microplate spectramax i3x molecular biocompareSpectramax i3. Absorbance Fluorescence (top/bottom read) Luminescence (top read) Time-Resolved Fluorescence (top read). . SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. Time Resolved Fluorescence: Solid white plates for top reads and white plates with clear bottoms for bottom reads. Spectranet customer portalThe SpectraMax® i3x Multi-Mode microplate reader measures spectral- based Absorbance, Fluorescence, and Luminescence on microplates that range from 6 to 384 wells. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. The StemoniX microBrain 3D platform is a homogenous 3D neural spheroid system in 96 and 384-well format. gen) on a Spectramax i3X luminometer. R8362. For wavelengths in the UV range above 220 nm,. 99. The chamber. Italic and boldface type are used for emphasis. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. For more detailed information and technical support of Life Technologies assays, please call 1-800-955-6288 and enter extension 40266 or email drugdiscoverytech@lifetech. The SpectraMax i3x is a multi-mode microplate reader that measures absorbance, fluorescence, and luminescence with user-configurable detection modules for western blot, cell imaging, and fast kinetics. Molecular Devices are known for both the reliability and the wide uses of their products. Open the catalog to page 1. Additionally, user-installable options allow the SpectraMax i3 System to grow with your changing application needs to fulfill and go beyond the standard reader applications, protecting your intial investment. Wokingham, Berkshire RG41 5TS United Kingdom. The SpectraMax i3x Multi-Mode Microplate Reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. . Example: [Setup]. Benefits • User-upgradeable application modules including cellular imaging • Sensitivity across spectrum with Spectral Fusion™ Illumination • Expanded dynamic range with cooled PMT • Control and analytics provided by SoftMax® Pro Software SpectraMax i3x The SpectraMax i3x from Molecular Devices is a multi-mode detection system that evolves. Determination of anti-inflammatory. The SpectraMax M4 reader had identical performance specifications to the SpectraMaxLuminescence was read on the SpectraMax i3x reader and the SpectraMax L reader. 5 decades, with sensitive detection of cells. Molecular Devices ScanLater Western Blot Detection Platform. The spectra analysis and K d were determined using Sigmaplot 14. . The SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. . For more sample throughput, the system canMicroplates were sealed to reduce evaporation (adhesive PCR plate seal; Thermo Fisher Scientific, AB0558) and placed in a microplate reader (Molecular Devices, SpectraMax i3x) for 8 h at 30 °C. 0´ 104 4. Faster data acquisition and analysis with preconfigured SoftMax Pro protocols. Supported Windows Operating System: See release notes. style. . Table 1. All test measurements and calculations are handledPreconfigured protocols and custom assay workflows simplify microplate data acquisition and analysis. (SpectraMax i3x and Paradigm readers only) SoftMax Pro Software SoftMax Pro® Software is the most published microplate reader control and microplate data analysis software package available to scientists.